Escala de competências socioemocionais para estudantes universitários: Produção de normas e sua relação com bem-estar subjetivo

As competências socioemocionais têm chamado a atenção de pesquisadores e programas de treinamento de habilidades socioemocionais têm sido desenvolvidos em muitos países. Este estudo teve como objetivo geral produzir normas para a escala de competências socioemocionais de estudantes universitários e analisar seu poder preditivo sobre o bem-estar subjetivo. A coleta foi realizada com uma amostra total de 1.020 estudantes de diversos cursos de graduação, de duas instituições de ensino particular do Distrito Federal. Para a coleta de dados foram utilizados dois instrumentos. O primeiro foi a escala de competências socioemocionais para estudantes universitários, de Souza, Faiad e Rueda (2021), que realiza a investigação de seis fatores. O segundo instrumento, destinado a mensuração do bem-estar subjetivo, foi composto pela escala de afetos positivos e negativos, de Zanon e Hutz (2014) e a escala de satisfação com a vida, de Zanon, Bardagi, Layous e Hutz (2013). Para análise dos dados foram realizados cálculos de correlações de Pearson e análises de modelos de regressão linear múltipla. Quanto aos resultados, normas para fins de diagnóstico foram produzidas para a população geral. Os resultados mostraram também que a maioria dos fatores de competências socioemocionais para estudantes universitários se correlacionaram significativamente com bem-estar subjetivo e foram capazes de predizer o bem-estar subjetivo. Concluiu-se que a escala de competência socioemocionais de estudante universitários pode ser utilizada para fins de diagnóstico, principalmente por apresentar um estudo normativo.

Socio-emotional skills have attracted the attention of researchers and training programs for socio-emotional skills have been developed worldwide. The general objective of this study was to produce norms for the socio-emotional competence scale of university students, as well as to analyze its predictive power on subjective well-being. The collection was carried out with a total sample of 1,020 students from different undergraduate courses, from two private educational institutions in the Federal District, in Brazil. Two instruments were used for data collection. The first was the Social-emotional Skills Scale for University Students, by Souza et al. (2021), which carries out the investigation of six factors. The second instrument, designed to measure subjective well-being, was composed of the Positive and Negative Affects Scale, by Zanon and Hutz (2014) and the Life Satisfaction Scale, by Zanon et al. (2013). For data analysis, calculations of Pearson correlations and analysis of multiple linear regression models were performed. As for the results, standards for diagnostic purposes were produced for the general population. The results also showed that most factors of socio-emotional competence for university students were significantly correlated with subjective well-being and others were able to predict subjective well-being. It is concluded that the Socio-emotional Competence Scale of University Students can be used for diagnostic purposes, mainly because it presents a normative study.

Las habilidades socioemocionales han llamado la atención de los investigadores y se han desarrollado programas de formación en habilidades socioemocionales en todo el mundo. El objetivo general de este estudio fue producir normas para la escala de competencia socioemocional de estudiantes universitarios, así como analizar su poder predictivo sobre el bienestar subjetivo. La recolección se realizó con una muestra total de 1.020 estudiantes de diferentes carreras de grado, de dos instituciones educativas privadas del Distrito Federal, en Brasil. Se utilizaron dos instrumentos para la recolección de datos. La primera fue la Escala de habilidades socioemocionales para estudiantes universitarios de Souza et al. (2021), que lleva a cabo la investigación de seis factores. El segundo instrumento, destinado a medir el bienestar subjetivo, estuvo compuesto por la Escala de afectos positivos y negativos, de Zanon y Hutz (2014) y la Escala de satisfacción con la vida, de Zanon et al. (2013). Para el análisis de datos, se realizaron cálculos de correlaciones de Pearson y análisis de modelos de regresión lineal múltiple. En cuanto a los resultados, se elaboraron estándares con fines diagnósticos para la población general. Los resultados también mostraron que la mayoría de los factores de competencia socioemocional para estudiantes universitarios se correlacionaron significativamente con el bienestar subjetivo y otros fueron capaces de predecir el bienestar subjetivo. Se concluye que la escala de competencia socioemocional de estudiantes universitarios puede ser utilizada con fines diagnósticos, principalmente porque presenta un estudio normativo.

Escala de Prosocialidad según Nivel de Cercanía (EPNC): Validación en Población Guanajuatense / Prosociality Scale according to Level of Closeness: Validation in Guanajuato Population

Resumen La conducta prosocial refiere a todas las conductas que tienen como finalidad ayudar a otra persona, institución o causa. Su estudio puede ser usado como factor protector ante conductas disruptivas (Auné et al., 2014; Correa, 2017; Richaud & Mesurado, 2016). Por lo que una escala que mida este constructo y además discrimine entre la conducta prosocial a personas cercanas y a personas que no lo son, resulta pertinente. El objetivo del presente estudio fue evaluar las características psicométricas de la Escala de Prosocialidad según nivel de cercanía (EPNC) en población adulta de la ciudad de León, Guanajuato. Participaron 323 personas (M= 32.52, D. E= 11.73), hombres y mujeres entre 18 a 60 años. Los resultados arrojaron una buena confiabilidad a través del alpha de Cronbach con un valor de .902; por medio de un análisis factorial de máxima verosimilitud con rotación ortogonal (varimax), se obtuvieron cuatro factores que en conjunto explican el 46.012% de la varianza. Se obtuvo un KMO de .888 y la prueba de esfericidad de Bartlett con un valor de 3278.753, p < 0,000. Dichos resultados concluyen que la escala cuenta con evidencias de validez de constructo y confiablidad para ser aplicada en población adulta de Guanajuato.

Abstract Prosocial behavior refers to all behaviors that are intended to help another person, institution, or cause. Its study can be used as a protective factor against disruptive behaviors (Auné et al., 2014; Correa, 2017; Richaud & Mesurado, 2016). Therefore, a scale that measures this construct and also discriminates between prosocial behavior towards close people and people who are not, is relevant. The objective of the present study was to evaluate the psychometric characteristics of the Prosociality Scale according to the level of closeness (EPNC by its acronym in Spanish) in the adult population of Leon, Guanajuato Mexico. 323 people participated (M= 32.52, SD= 11.73), men and women between 18 and 60 years old. The results yielded adequate Cronbach's alpha values (.902); through a maximum likelihood factor analysis with orthogonal rotation (varimax), four factors were obtained that together explain 46.012% of the variance. A KMO of .888 was obtained and the Bartlett sphericity test with a value of 3278.753, p < 0.000. These results conclude that the scale has evidence of construct validity and reliability to be applied in the adult population of Guanajuato.

Opiniones y recomendaciones de la Sociedad Argentina de Diabetes. Hemoglobina A1c / Opinions and recommendations of the Argentine Diabetes Society. Hemoglobin A1c

La estandarización de la hemoglobina A1c (HbA1c) permitió en algunos países su uso para el diagnóstico de la diabetes mellitus (DM) y la prediabetes, además de su empleo en el seguimiento del paciente con DM. Es importante recordar que se trata de una medida indirecta del promedio glucémico durante el tiempo de vida media del eritrocito, pudiendo verse afectada por variables no glucémicas, como también por interferencias analíticas según la metodología empleada para su determinación. A continuación, se describen las recomendaciones y consideraciones a tener en cuenta para la determinación de la HbA1c cuando se emplea como criterio diagnóstico de la DM, teniendo en cuenta que al utilizarla para tal fin es necesario que la medida se realice con métodos certificados y estandarizados al ensayo utilizado en el Diabetes Control and Complications Trial (DCCT).

The standardization of hemoglobin A1c (HbA1c) allowed in some countries its use for the diagnosis of diabetes mellitus (DM) and prediabetes, in addition to its use in the follow-up of patients with DM. It is important to highlight that it is an indirect measurement of the glycemic average during the halflife of the erythrocyte, and may be affected by non-glycemic variables, as well as by analytical interferences depending on the methodology used for its determination. The recommendations and considerations to take into account for the determination of HbA1c when it is used as a diagnostic criterion for diabetes are described below. In addition, it is important to emphasize that it is essential that the HbA1c measurement be performed with certified and standardized methods to the Diabetes Control and Complications Trial (DCCT)results.

Exploring the Historical Background and Clinical Implications of Electrocardiogram in the Context of Chagas Disease Research

ABSTRACT Chagas disease (CD) remains one of the most significant endemic diseases in Latin America. Approximately 30% of individuals with CD develop the cardiac form, the main determinant of morbidity and mortality, which is characterized by typical electrocardiogram (ECG) changes caused by chronic chagasic cardiopathy (CCC). This review accentuates to how crucial it is for research teams and reference centers that treat patients with CD to standardize ECG in CCC. This was a non-systematic review of the literature. ECG is the most widely used examination in the diagnosis and evaluation of CCC, and it is also employed in epidemiological surveys, risk stratification for cardiovascular events and death, and monitoring the clinical progression of the disease. Carlos Chagas and Eurico Villela published the first work addressing CCC in 1922. Other works followed, including the study by Evandro Chagas' which was the first to perform ECG in CD, culminating in Francisco Laranja's seminal work in 1956. Since the 1980s, standardizations and ECG reading codes for CD have been established. This standardization aimed to code complex arrhythmias and characteristic ventricular conduction disorders and standardize ECG readings for clinical and epidemiological studies in CD. Nearly all existing electrocardiographic abnormalities can be found in CD, with a predominance of abnormalities in the formation and conduction of cardiac stimuli. The complex and heterogeneous substrate of CD with varied electrocardiographic manifestations poses a significant challenge when comparing studies involving patients with CCC, emphasizing the need for ECG standardization in CD.

Dosimetry standardization in radiobiological research / 中华放射肿瘤学杂志

In recent years, the issue of "reproducibility" of scientific research results has become more and more prominent. Radiobiology is a medical science that studies the biological effect of radiation on living organisms, and there is also a serious problem of "reproducibility of findings". Inaccuracy of physical dose or incomplete dosimetric reports is one of the main causes. Use of guidelines, specifications and recommendations for dosimetric measurement, such as the standardized scoring system for dosimetric reports, will help improving the standardization and accuracy of physical dose measurement in radiobiological research. In this article, multiple guidelines and recommends for improving collaboration between radiobiology and radiation physics, as well as for dose standardization of radiobiological research were evaluated, aiming to provide reference for improving the reproducibility of radiobiological research.

Expert consensus on RhD blood group testing strategies in blood transfusion compatibility testing laboratories / 中国输血杂志

RhD antigen is the most immunogenic antigens in the Rh blood group system and can cause hemolytic transfusion reactions (HTRs) and severe hemolytic disease of the fetus and newborn (HDFN). RhD-negative red blood cell (RBC) is a scarce resource in China, and RhD-negative patients face challenges in timely blood transfusion. For different populations that were initially negative for RhD (saline method), what techniques should be used, how to issue test report and when should " Asian type" DEL (RHD*01EL.01, c.1227G>A) be identified are the confusions of blood transfusion workers in the process of clinical blood supply. More than 200 technical experts discussed and reached a consensus on blood transfusion compatibility testing for Chinese population. The purpose of the consensus is to further standardize and refine the RhD blood group testing strategy, provide technical support for achieving accurate blood transfusion for patients with different RhD blood groups, continuously improve the levels of prevention and treatment for RhD-HTRs and RhD-HDFN, and lay a foundation for the future development of industry standards concerning RhD blood group testing strategy.

Research progress of platelet lysates in the field of regenerative medicine and cell therapy / 中国输血杂志

Human platelet lysates(HPL), as a new type of biomaterial, can promote tissue repair, cell proliferation and inflammation control. This paper introduced the development of HPL in the field of regenerative medicine and cell therapy and summarizes their application. The potential of HPL to promote cell proliferation was used as an entry point to show its advantages as a supplement of cell culture medium. Since there is currently no standard procedure for HPL preparation, this paper sorts out the standardization elements such as raw materials source, donor variability and preparation technology, in order to provide reference for the establishment of standards of relevant industry in the future.

Application of business contract audit in the management of multi-campus hospitals / 中华医院管理杂志

With business activities expanding and number of business contracts growing, the management of multi-campus hospitals is faced with constantly escalating requirements for risk prevention and control within the hospital. Being an important part of hospital internal management, the internal audit plays an irreplaceable role in the high-quality development of hospitals. The authors elaborated on the value of business contract audit in the management of multi-campus hospitals from three dimensions of internal control, risk management and hospital added value, introduced the practices by the Second Affiliated Hospital Zhejiang University School of Medicine in the management of its multi-campus management since 2018, by adopting the strategy of architecture integration, rule homogenization, process standardization, and vertical supervision, and put forward suggestions from three aspects, including the practical difficulties in the participation process, the dynamic adjustment of role positioning, and the development of better integrating business contract auditing into multi-campus hospital management.

Systolic blood pressure variation within a visit of community hypertension patients in Shanghai / 上海预防医学

ObjectivesTo investigate characteristics and influencing factors of short-term variation of systolic blood pressure of community hypertension patients in Shanghai. MethodsBased on the standardized blood pressure measurement data of hypertensive patients in Shanghai （2018‒2021） and the noninfectious chronic disease management system database， coefficient of variation of systolic blood pressure （CV_S） was described and the influencing factors were analyzed. ResultsAmong 112 680 community hypertension patients， males accounted for 46.87% with a median age of 69 years. CV_S was 0.038 6 ± 0.035 0. Generalized linear model analysis showed the following influencing factors of CV_S： gender， females were higher than males， B=0.032， P<0.001； age group， 60‒69 years， 70‒79 years old group， and ≥80 years groups were all higher than <50 years group， B=0.042， 0.056， and 0.074 respectively with P values of 0.020， 0.002， and <0.001 respectively； smoking， smoking cessation and smoking everyday was lower than never smoking， B=-0.032 and-0.028 respectively with P values of 0.023 and 0.007 respectively； systolic blood pressure， 140‒159 mmHg group， 160‒179 mmHg group， and ≥180 mmHg group were lower then <140 mmHg group， B=-0.039， -0.091， and -0.175 respectively with P values all <0.001； and measurement season， autumn was lower than spring， （B=-0.056， P<0.001）. The paired test showed that CV_S calculated from the three measurements was 1.04% higher than that calculated from the two measurements （P<0.001）. ConclusionThe coefficient of variation of systolic blood pressure of community hypertension patients in Shanghai has large variation， and was influenced by many factors.

Historical investigation on paradigm of "species standardization" of Chinese herbal medicine / 中国中药杂志

The paradigm can be summarized as the same methodological principles followed by the members of the learning community who received common education, training, and edification in the common social and historical stage, and had common academic views, concepts, and terms. The "species standardization" paradigm of botanical drugs in China has experienced the period of non-standardization of names(the end of the Warring States period), the period of multi-dimensional textual research paradigm of traditional materia medica(from the end of the Warring States period to 1930s), and periods of modern plant taxonomy system research paradigm(from 1930s to the present). The multi-dimensional textual research paradigm of traditional materia medica is characterized by the textual research of species in different regions and years based on the classification of the natural attributes and the habit of correspondence between names and materials, from the multi-dimensional investigation of the name, appearance, color, smell, texture, growth environment, efficacy, and morphological characteristics of medicinal materials, to find the correspondence between names and materials. The purpose of the progress in traditional species standardization is practicality for humans, and there is a lack of systematic research on the classification and naming principles in line with its laws from the plant itself, resulting in inaccurate research results. The research paradigm of modern plant taxonomy is herbal research. The characteristics of this paradigm are using plant taxonomy knowledge to conduct textual research to confirm the species of the drug and calibrate the scientific name by the double name method, based on the plant morphology, harvest season, origin distribution, ecological habits, names, drug efficacy, and other data in the lite-rature of previous dynasties. This paradigm combines the knowledge of traditional materia medica and modern plant taxonomy, and the results of species research are accurate.

Application and research progress of ultrasound in acupuncture treatment / 中国针灸

The operation standardization, quantitative standard and safety of acupuncture treatment are important links in the development of acupuncture modernization. In recent years, with the continuous development of ultrasonic imaging technology, ultrasonic medicine has the characteristics of visualization, quantitative analysis and real-time dynamics, which could play a unique role in acupuncture treatment. In this paper, the research progress of the combined application of ultrasonic medicine and acupuncture treatment is described from three aspects: ultrasound guidance helping to standardize acupuncture operation, ultrasound guidance helping to improve and evaluate the clinical efficacy of acupuncture, and ultrasound guidance helping to improve the safety of acupuncture, aiming to providing new ideas for the application of modern medicine in traditional medicine.

Characterization and Comparison Analysis of WHO Monographs on Selected Medicinal Plants and ISO Standards of Chinese Materia Medica / 中国结合医学杂志

Medicinal plants or Chinese materia medica (CMM) are now attracting worldwide attention as they have increasingly prominent advantages over chemical drugs in disease treatment and healthcare. Since the 1990s, World Health Organization (WHO) and International Organization for Standardization established the Technical Committee of Traditional Chinese Medicine (ISO/TC 249) have carried out the development of quality standards on medicinal plants or CMMs respectively, and a considerable number of monographs and international standards have been published. Since the two international organizations adhere to different principles, the standards they develop naturally have different emphasis. Driven by market demand and international trade, ISO mainly takes quality, efficacy and safety into consideration when developing standards, while WHO pays more attention to clinical practice, quality control and medication guidance. Up to now, there is a lack of comparative analysis on the records, background, principles, basic content, and main requirements of quality standards on medicinal plants or CMMs respectively published by WHO and ISO. Therefore, based on international standards of CMM developed by ISO/TC 249 platform and WHO Monographs on Selected Medicinal Plants, this paper systematically compares the purposes, selected principles, standard-developing process, basic content, and main quality requirements to summarize their similarities and differences, and find their merits, aiming to serve as a reference to the development of international standards for CMMs that helps them go global.

Contributions of the institutions for the nursing professionalization: integrative review (2010-2020) in the light of freidsonian conceptions / Contribuciones de las entidades para la profesionalización de la enfermería: revisión integradora (2010-2020) bajo la luz de las concepciones freidsonianas / Contribuições das entidades para profissionalização da enfermagem: revisão integrativa (2010-2020) à luz das concepções freidsonianas

ABSTRACT Objective: To analyze the contributions of the Brazilian Nursing Association and the Federal and Regional Nursing Councils in the literature for the professionalization of nursing in the light of Eliot Freidson's theoretical conceptions. Methods: Integrative review of the literature, of socio-professional historical interest, carried out from June to November 2021, through the question: How did professional associations contribute to the professionalization of Brazilian nursing according to the literature from 2010 to 2020? The evidence were organized in a synoptic table, which allowed the construction of a conceptual map. Results: In the 23 selected studies, the professional associations presented scientific, social and political contributions, which shape the professional field of nursing, enabling knowledge/expertise, autonomy and self-regulation. Final considerations: The efforts of these institutions reaffirm nursing as an academic and consulting profession, capable of exerting control over the essence of nursing work. They strive to consolidate nursing as a legitimate professional field of health in Brazil.

RESUMEN Objetivo: Analizar las contribuciones de la Asociación Brasileña de Enfermería y de los Consejos Federales y Regionales de Enfermería a la profesionalización de la enfermería bajo la luz de las concepciones teóricas de Eliot Freidson Métodos: Se trata de una revisión integradora, de interés histórico socio profesional, realizada de junio a noviembre de 2021, mediante la pregunta: ¿Cómo han contribuido las entidades de clase con la profesionalización de la enfermería brasileña según la literatura de 2010 a 2020? Las pruebas se organizaron en un cuadro sinóptico que permitió construir un mapa conceptual. Resultados: En los 23 estudios seleccionados, las entidades de clase presentaron contribuciones científicas, sociales y políticas, que forman el campo profesional de la enfermería y facilitan el conocimiento, la experticia, la autonomía y la autorregulación. Consideraciones finales: Las entidades reafirman que la enfermería es una profesión académica y de consulta, capaz de ejercer el control sobre la esencia del trabajo en sí y perseveran para consolidarla como un campo profesional legítimo en el Brasil.

RESUMO Objetivo: Analisar na literatura contribuições da Associação Brasileira de Enfermagem e dos Conselhos Federal e Regionais de Enfermagem para a profissionalização da enfermagem à luz das concepções teóricas de Eliot Freidson. Métodos: Revisão integrativa, de interesse histórico socioprofissional, realizada de junho a novembro de 2021, mediante a questão: Como as entidades de classe contribuíram para a profissionalização da enfermagem brasileira de acordo com a literatura de 2010 a 2020? Organizaram-se as evidências em quadro sinótico, que possibilitou a construção de mapa conceitual. Resultados: Nos 23 estudos selecionados, as entidades de classe apresentaram contribuições científicas, sociais e políticas, que conformam o campo profissional da enfermagem, possibilitando conhecimento/expertise, autonomia e autorregulação. Considerações finais: Os esforços dessas entidades reafirmam a enfermagem como profissão acadêmica e de consulta, capaz de exercer controle sobre a essência do trabalho da enfermagem. Elas se empenham em consolidar a enfermagem como legítimo campo profissional da saúde no Brasil.

Avaliação de perguntas e a interação entrevistador-entrevistado em entrevista com uso de questionário padronizado / Evaluating questions and interviewer-interviewee interaction in an interview using a standardized questionnaire

Resumo A avaliação de perguntas de questionário de inquérito de saúde no Brasil limita-se, na maioria das vezes, aos testes-piloto e debriefing das entrevistas. O objetivo do presente artigo é contribuir para preencher essa lacuna ao apresentar uma metodologia de avaliação cognitiva para identificação de perguntas potencialmente "problemáticas" quanto à sua formulação e o que ocorre, na interação entrevistador-entrevistado, ao aplicá-las em entrevistas com o uso de questionário padronizado em um inquérito regional de saúde. O estudo utilizou dois roteiros padronizados para identificação de dificuldades de redação e/ou compreensão e avaliação da interação entrevistador-entrevistado. Dentre as 513 perguntas do questionário individual de pesquisa, 68 questões foram indicadas como sendo potencialmente problemáticas à compreensão e comunicação pelos entrevistados. As perguntas que apresentaram problemas na sua formulação, com maior pontuação (>40), foram aquelas relacionadas à quantificação de tempo; ao grau de dificuldade para o autocuidado; à situação no trabalho e à estimação do rendimento. Dentre as perguntas apontadas como problemáticas na avaliação pelo Sistema de Apreciação de Perguntas (SAP), o autocuidado e horas trabalhadas estão entre aquelas que também tiveram pontuação alta na avaliação da interação.

Abstract The evaluation of health survey questions in Brazil is limited, in most cases, to pilot tests and interview debriefing. The purpose of this article is to contribute to filling this gap by presenting a cognitive assessment methodology for identifying potentially "problematic" questions regarding their formulation and what happens in the interviewer-interviewee interaction, when applying them in interviews using the standardized questionnaire in a regional health survey. In this study, two standardized scripts were used to identify difficulties in writing and/or understanding and evaluating the interviewer-interviewee interaction. Among the 513 questions in the individual research questionnaire, 68 questions were indicated as being potentially problematic for the interviewees to understand and communicate. The questions that presented problems in their formulation, with the highest score (>40), were those related to the quantification of time; the degree of difficulty for self-care; work situation and income estimation. Among the questions identified as problematic in the evaluation by the Question Appraisal System (SAP), self-care and hours worked are among those that also scored high in the interaction evaluation.

Estandarización de un ensayo de adhesión de Escherichia coli para el cribado de agentes antibiopelículas / Standardization of an Escherichia coli adhesion assay for anti-biofilm agents screening

Introducción: Las biopelículas constituyen un factor clave en el desarrollo de enfermedades infecciosas y la resistencia a fármacos para su control. Nuevas estrategias terapéuticas incluyen productos naturales como agentes antibiopelículas. Sin embargo, la comparación de los resultados suele ser difícil debido a la falta de homogeneidad y estandarización en los métodos empleados para estudiar la formación de biopelículas in vitro. Objetivo: Estandarizar un ensayo de adhesión en microplaca de Escherichia coli para su uso en el cribado de potenciales agentes antibiopelículas. Métodos: Se utilizó el método de adherencia en microplaca y la tinción con violeta cristal. Se evaluó la influencia de condiciones experimentales como la concentración bacteriana, el medio de cultivo y el tiempo de incubación. Resultados: Se identificaron como condiciones óptimas para la formación de biopelículas: el medio Luria Bertani (LB), la concentración bacteriana a 105 UFC/mL y un tiempo de incubación de 24 h. Conclusiones: Los resultados mostraron que las condiciones de cultivo influyen en la formación de biopelículas. Se determinaron las condiciones de cultivo óptimas para la formación de biopelículas de E. coli, que podrían emplearse en futuros estudios acerca del efecto de productos naturales sobre la inhibición o destrucción de biopelículas.

Introduction: Biofilms are a key factor in the development of infectious diseases and drug resistance for their control. New therapeutic strategies include natural products as anti-biofilm agents. However, comparing results is often difficult due to the lack of homogeneity and standardization of the methods used to study biofilm formation in vitro. Objective: To standardize an Escherichia coli microplate adhesion assay for potential anti-biofilm agents screening. Methods: The microplate adhesion method and crystal violet staining were used. The influence of experimental conditions such as bacterial concentration, culture medium, and incubation time were evaluated. Results: Optimal conditions for biofilm formation included: Luria Bertani (LB) medium, bacterial concentration at 105 CFU/mL, and an incubation time of 24 hours. Conclusions: The results showed that culture conditions influence biofilm formation. Optimal culture conditions for the formation of E. coli biofilms were determined, which could be used in further studies on the effect of natural products on the inhibition or destruction of biofilms.

Determinação do período de estabilidade de soluções volumétricas rotineiramente utilizadas em laboratórios de análises químicas / Determination of the stability period of volumetric solutions routinely used in chemical analysis laboratories / Determinación del período de estabilidad de las soluciones volumétricas utilizadas habitualmente en los laboratorios de análisis químicos

As soluções volumétricas são rotineiramente utilizadas nos laboratórios, principalmente nos processos de síntese de produtos e nas análises quantitativas de matéria-prima e/ou produto acabado, entretanto poucos são os estudos que abordam a estabilidade destas soluções. Considerando que a qualidade das soluções volumétricas pode afetar os procedimentos de análises químicas e consequentemente induzir a erros, e ainda que, a Farmacopeia Brasileira (2010) não cita tempo máximo de utilização dessas soluções padronizadas, a avaliação da estabilidade das mesmas é importante. Sendo assim, o objetivo do trabalho foi avaliar a estabilidadede 10 soluções volumétricas, empregadas rotineiramente em laboratórios de análises químicas, com o intuito de estabelecer o período que essas soluções permanecem estáveis, isto é, sem sofrer alteração na concentração. As metodologias de preparo e padronização das soluções volumétricas seguiram os métodos descritos na Farmacopeia Brasileira (2010), sendo as mesmas padronizadas no momento do preparo e a cada 20 dias, por um período de 180 dias. As soluções contendo ácidos e bases, bem como as soluções de iodato de potássio e nitrato de prata, permaneceram constantes durante o período de análises. As soluções de EDTA, iodo, nitrito de sódio, permanganato de potássio e tiossulfato de sódio apresentaram estabilidade inferior a 180 dias, tornando necessária a realização de padronização periódica. As soluções volumétricas utilizadas nos laboratórios apresentam diferentes estabilidades, o que ressalta a importância da determinação do período que as mesmas se mantêmcom as concentrações estáveis, evitando possíveis alterações de resultados nas análises químicas.

Volumetric solutions are routinely used in laboratories, mainly in product synthesis processes and in quantitative analyzes of raw materials and/or finished products, however there are few studies that address the stability of these solutions. Considering that the quality of volumetric solutions can affect chemical analysis procedures and consequently induce errors, and even though the Brazilian Pharmacopoeia (2010) does not mention the maximum time for using these standardized solutions, the evaluation of their stability is important. Therefore, the aim of this work was to evaluate the stability of 10 volumetric solutions, routinely used in chemical analysis laboratories, in order to establish the period that these solutions remain stable without changing their concentrations. The methodologies for preparing and standardizing the volumetric solutions followed the methods described in the Brazilian Pharmacopoeia (2010), being standardized at the time of preparation and every 20 days, for a period of 180 days. Solutions containing acids and bases, as well as potassium iodate and silver nitrate solutions, were stable during the analysis period. The solutions of EDTA, iodine, sodium nitrite, potassium permanganate and sodium thiosulfate showed stability less than 180 days, making it necessary to carry out periodic standardization of these solutions. The volumetric solutions used in the laboratories have different stabilities, which highlights the importance of determining the period in which they remain stable, avoiding possible changes in results in chemical analyzes.

Las soluciones volumétricas se utilizan de forma rutinaria en los laboratorios, principalmente en los procesos de síntesis de productos y en el análisis cuantitativo de materias primas y/o productos acabados. Sin embargo, existen pocos estudios que aborden la estabilidad de estas soluciones. Considerando que la calidad de las soluciones volumétricas puede afectar los procedimientos de análisis químico y consecuentemente inducir a errores, y también que, la Farmacopea Brasileña (2010) no menciona el tiempo máximo de uso de estas soluciones estandarizadas, la evaluación de su estabilidad es importante. Así, el objetivo del trabajo fue evaluar la estabilidad de 10 soluciones volumétricas, utilizadas rutinariamente en los laboratorios de análisis químico, con el fin de establecer el período en que estas soluciones permanecen estables, es decir, sin sufrir alteraciones en la concentración. Las metodologías de preparación y estandarización de las soluciones volumétricas siguieron los métodos descritos en la Farmacopea Brasileña (2010), siendo las mismas estandarizadas en el momento de la preparación y cada 20 días, por un período de 180 días. Las soluciones que contienen ácidos y bases, así como las soluciones de yodato de potasio y nitrato de plata, permanecieron constantes durante el periodo de análisis. Las soluciones de EDTA, yodo, nitrito de sodio, permanganato de potasio y tiosulfato de sodio fueron estables durante menos de 180 días, por lo que fue necesario realizar estandarizaciones periódicas. Las soluciones volumétricas utilizadas en los laboratorios presentan diferentes estabilidades, lo que pone de manifiesto la importancia de determinar el periodo que permanecen con concentraciones estables, evitando posibles cambios en los resultados en los análisis químicos.

Las normas técnicas para la formación en ingeniería biomédica, tecnología y administración en salud / Technical standards for the training in biomedical engineering, health technology and health management

Introducción: Los documentos normativos establecen el estado del arte relacionado con determinado campo del conocimiento. Existe una gran cantidad de normas relacionadas con los servicios de salud y su gestión, cuya aplicación es relevante en este sector. Objetivo: Exponer la importancia de las normas técnicas en la formación de los profesionales en ingeniería biomédica, tecnología y administración en salud. Desarrollo: Diferentes aspectos relacionados con el desempeño y las funciones de los profesionales en ingeniería biomédica, tecnología de la salud y administración en salud están recogidos en normas técnicas internacionales y en otras de carácter nacional, que resultan pertinentes y de gran utilidad para su formación en el nivel de grado y el posgrado. Conclusiones: Las profesiones abordadas requieren emplear los documentos normativos relacionados con sus funciones para contribuir con la calidad de los servicios de salud; de ahí la pertinencia de su incorporación en los planes de estudio de estas carreras(AU)

Introduction: Normative documents establish the state of the art related to a certain field of knowledge. There is a large number of standards related to health services and their management, whose application is relevant in this sector. Objective: To show the importance of technical standards in the training of professionals from the fields of biomedical engineering, health technology and health management. Development: Different aspects related to the performance and functions of professionals from the fields of biomedical engineering, health technology and health management are gathered in international and other national technical standards, relevant and useful for their training at the undergraduate and postgraduate levels. Conclusions: The addressed professions require the use of normative documents related to their functions in order to contribute to the quality of health services, hence the relevance of their incorporation into the curriculums of these major(AU)

Las normas técnicas para la formación en ingeniería biomédica, tecnología y administración en salud / Technical standards for the training in biomedical engineering, health technology and health management

Introducción: Los documentos normativos establecen el estado del arte relacionado con determinado campo del conocimiento. Existe una gran cantidad de normas relacionadas con los servicios de salud y su gestión, cuya aplicación es relevante en este sector. Objetivo: Exponer la importancia de las normas técnicas en la formación de los profesionales en ingeniería biomédica, tecnología y administración en salud. Desarrollo: Diferentes aspectos relacionados con el desempeño y las funciones de los profesionales en ingeniería biomédica, tecnología de la salud y administración en salud están recogidos en normas técnicas internacionales y en otras de carácter nacional, que resultan pertinentes y de gran utilidad para su formación en el nivel de grado y el posgrado. Conclusiones: Las profesiones abordadas requieren emplear los documentos normativos relacionados con sus funciones para contribuir con la calidad de los servicios de salud; de ahí la pertinencia de su incorporación en los planes de estudio de estas carreras(AU)

Introduction: Normative documents establish the state of the art related to a certain field of knowledge. There is a large number of standards related to health services and their management, whose application is relevant in this sector. Objective: To show the importance of technical standards in the training of professionals from the fields of biomedical engineering, health technology and health management. Development: Different aspects related to the performance and functions of professionals from the fields of biomedical engineering, health technology and health management are gathered in international and other national technical standards, relevant and useful for their training at the undergraduate and postgraduate levels. Conclusions: The addressed professions require the use of normative documents related to their functions in order to contribute to the quality of health services, hence the relevance of their incorporation into the curriculums of these major(AU)

Estandarización de un procedimiento espectrofotométrico para la cuantificación de polisacárido capsular de Neisseria meningitidis serogrupo X / Standardization of a spectrophotometric procedure for the quantification of Neisseria meningitidis serogroup X capsular polysaccharide

En el presente trabajo se realiza la estandarización del procedimiento espectrofotométrico de determinación de polisacárido capsular e intermedios de Neisseria meningitidis serogrupo X, mediante la determinación de los grupos fosfodiéster presentes en su estructura, por el método de Chen. Se realizó un análisis de los siguientes criterios para la estandarización: linealidad, precisión (repetibilidad y precisión intermedia) y exactitud. Se demostró mediante el diseño experimental y los procedimientos estadísticos empleados que el método es lineal (r > 0,99), el coeficiente de variación del factor respuesta < 5 por ciento, la desviación estándar relativa de la pendiente < 2 por ciento, no existiendo diferencia estadísticamente significativa entre el intercepto de la ecuación con respecto a cero; exacto, porque no existe diferencia estadísticamente significativa entre la concentración determinada en un material de trabajo y su concentración nominal; también demostró ser repetible, pues el coeficiente de variación de las concentraciones de la muestra evaluada (2,44; 2,43; 0,88 por ciento para las concentraciones bajas, medias y altas, respectivamente) es inferior al 3 por ciento y no existen diferencias estadísticamente significativas entre las medias de los resultados obtenidos por dos analistas, evaluados durante cuatro días a tres niveles de concentración. La precisión intermedia es satisfactoria(AU)

The present work comprises the standardization a spectrophotometric procedure for assessing Neisseria meningitidis, serogroup X capsular polysaccharide and their intermediates of modification, the phosphodiesters groups present in its structure, based on Chen method. An analysis of the following standardization criteria was performed: linearity, precision (repeatability and intermediate precision) and accuracy. It was demonstrated through the experimental design and the statistical procedures used that the method is linear (r > 0.99), the coefficient of variation of the response factor < 5 percent, the relative standard deviation of the slope < 2 percent, with no statistically significant difference between the intercept of the equation with respect to zero; exact, because there is no statistically significant difference between the concentration determined in a work material and its nominal concentration; it also proved to be repeatable, because the coefficient of variation of the concentrations of the sample (2.44; 2.43; 0.88 percent for low, medium and high concentrations respectively) is less than 3 percent and there is no statistically significant difference between the means of the results obtained by two analysts, evaluated for four days at three concentration levels. Its intermediate precision was satisfactory(AU)

Teste de inteligência não verbal R-1: o tamanho do efeito para as normas de 2018 / R-1 non-verbal intelligence test: the effect size for the 2018 standards

O estudo apresenta os tamanhos do efeito, seus intervalos de confiança, classificações e visualizações para a atualização normativa de 2018 do Teste não verbal de Inteligência R-1, visto que as diferenças estatisticamente calculadas com base no valor p oferecerem evidências tênues contra a hipótese nula e, por isso, não servem como uma prova da significância clínica. Utilizou-se a amostra normativa do instrumento, composta por 5.595 adultos, de ambos os sexos, com idades entre 18 e 65 anos, provenientes das cinco regiões geográficas brasileiras e subdivididos pelos níveis de ensino fundamental, médio e superior. Os resultados apontaram tamanhos do efeito entre médios e grandes na comparação entre os níveis de ensino fundamental e médio; grande a muito grande na relação entre o ensino fundamental e o superior e; pequenos a médios entre os níveis médio e superior de ensino. Concluiu-se que as diferenças estatisticamente observadas, por meio das análises de variância e pos-hoc de Tukey refletem um efeito real quando se considera o impacto da variável nível de escolaridade no desempenho do teste. Entendeu-se que os estudos normativos do instrumento refletem um impacto real quando se considera a variável em estudo, devendo incluir o tamanho do efeito e seus respectivos intervalos de confiança.

The study presents the effect sizes, their confidence intervals, ratings, and visualizations for the 2018 normative update of the R-1 Nonverbal Intelligence Test, since the statistically calculated differences based on the p-value offer tenuous evidence against the null hypothesis and do not serve as a proof of clinical significance. We used the normative sample of the instrument, consisting of 5,595 adults of both sexes, aged 18 to 65 years, from the five Brazilian geographic regions and subdivided by elementary, middle, and high school levels. The results showed medium to large effect sizes in the comparison between elementary and high school levels; large to very large in the relation between elementary and high school; and small to medium effect sizes between high school and high school levels. It was concluded that the statistically observed differences, by means of variance and Tukey's post-hoc analyses reflect a real effect when considering the impact of the education level variable on test performance. It was understood that normative studies of the instrument reflect a real impact when considering the variable under study, and should include the effect size and their respective confidence intervals.